About Our Lab

The Cardiovascular Physiology Core Facility will provide University of Wisconsin (UW) and non-UW researchers with a central resource for creating and studying animal models of disease on a fee-for-service basis. While the central focus of the facility is cardiovascular research, the techniques employed are often useful to investigators in other fields. Currently, the facility is conducting studies in cancer, bladder/prostate disease, drug toxicology, and gene/RNAi therapy in liver and skeletal muscle, as well as cardiovascular disease. One of the most popular services provided by the facility is evaluation of the cardiovascular phenotype of transgenic mice.

Mission

The Mission of the CVRC Physiology and Surgery Core Laboratory is to provide a central resource of knowledge, skills, personnel, and equipment that allows investigators access to cardiovascular and other techniques to facilitate research.

Staff and Facilities

Cardio Core is staffed by three experienced, full-time professionals and located in 1000 square feet of laboratory, surgical space and office space on the first floor of the Clinical Science Center (600 Highland Ave, Madison, WI  53792).

The facilities include:
• Fully equipped small animal operating theaters
• State-of the art echocardiography
• Mouse ECG and blood pressure monitoring via telemetry
• Pressure volume analysis for small animals
• Exercise testing and training
• Physiologic recording equipment
• Sonomicrometry
• Langendorff and working heart apparatus
• Fully equipped large animal operating theater
• Fluoroscopy
• State-of the art echocardiography
• Pressure volume analysis for large animals
• Swan-Ganz catheters – measuring right heart pressure and cardiac output
• Implantable loop recorders for chronic ECG monitoring
• Large animal telemetry (ECG, flow and pressure)
• Complete patient monitoring (EtCO2, pressure, ECG, Pulse Ox)
• Access to MRI, CT, PET for additional imaging.

Services

Services are oriented towards providing investigators with data suitable for use in a grant proposal or manuscript. Staff works with clients to assess their needs and provide the appropriate level of technical and scientific assistance. In addition to the techniques listed below, other techniques may be available upon request.

Mice and Rats

• Models of chronic myocardial infarction, heart failure and cardiac hypertrophy
• Evaluation of cardiac mechanics using closed chest measurement of pressure and volume via admittance catheter
• Exercise tolerance testing and exercise training
• Ambulatory blood pressure and ECG recordings (telemetry)
• Echocardiographic and Doppler imaging
• Stroke models
• Chronic indwelling catheters

Dogs, Pigs and Non-Human Primates

• Acute, open-chest porcine models of myocardial ischemia, infarction and stunning
• Acute open- and closed-chest canine and porcine preparations for investigating coronary blood flow, myocardial metabolism and arrhythmias
• Chronic canine and porcine models of myocardial ischemia, stunning, hibernation and heart failure
• Complete echocardiographic and Doppler imaging